Metabolomics

Share

Aa-+

The laboratory's main research themes, under the supervision of Dr. Christine Des Rosiers:

Factors involved in the development and progression of heart disease: Role of metabolic abnormalities and their modulation by pharmacological agents or diet.
Discovery of new biomarkers through metabolomics: Cardiovascular, mitochondrial and inflammatory diseases.

Laboratory expertise:

Mass spectrometry-targeted and -non-targeted  metabolomics analyses (gas and liquid chromatography: GC-MS, LC-MS/MS) applied to various classes of metabolites: phospholipids, cholesterol, sphingolipids, acylcarnitines, bile acids, fatty acids, organic acids and amino acids.

Metabolic and functional phenotyping of the working mouse heart through ex vivo perfusion in the presence of carbon-13 labelled substrates.
Metabolic investigations: analysis of metabolic flux based on the use of substrates with stable isotopes and mass spectrometry.

Team members

Students/post-doctoral fellows:

Sandra Therrien, BSc, M.Sc. Bioinformatics: Metabolomics and medecinal drugs response prediction in intestinal inflammatory disease: Development of a bioinformatics tool to link genes to metabolites.

Yvette Mukaneza, Ph.D. candidate nutrition, co-supervision with Dr. Lise Coderre: Impact of the mutation of the LRPPRC gene on AMP-activated protein kinase (AMPK) in the fibroblasts of patients with Leigh Syndrome French Canadian type (LSFC).

Matthieu Ruiz, PhD, Postdoctoral Fellow, co-supervision with Dr. Bruce Allen: Potential cardioprotective effect of the deletion of MAPK/MK2 in the development of diabetic cardiomyopathy.

Research assistants and associates:

Bertrand Bouchard, MSc Microbiology: Expertise in mass spectrometry (GC-MS and LC-MS/MS), including sample preparation; Email: bertrand.bouchard@mhi-rc.org

Lise Coderre, PhD Molecular Biology: Expertise in metabolic syndrome and diabetes, molecular signalling; Email: lise.coderre@umontreal.ca

Caroline Daneault, MSc Biochemistry: Expertise in mass spectrometry (GC-MS), results, data and sample analysis/preparation; Email: caroline.daneault@icm-mhi.org

Anik Forest, MSc Physics and Chemistry: Expertise in mass spectrometry (GC-MS/LC-MS/MS) and results analysis (“data mining”); Email: anik.forest@mhi-rc.org

Isabelle Robillard Frayne, MSc Chemistry: Expertise in mass spectrometry (GC-MS), results, data and sample analysis/preparation; Email: isabelle.robillard-frayne@icm-mhi.org

Julie Thompson Legault, MSc Nutrition: Expertise in metabolomics and clinical study coordination; Email: julie.thompson@mhi-rc.org

Research projects

The laboratory's main on-going research projects are funded by peer-review agencies, such as the Canadian Institutes of Health Research (CIHR), Genome Canada/Génome Québec, and the Canada Foundation for Innovation (CFI)

  1. “Metabolic investigations using mass isotopomer analysis: A paradigm for biomarker and therapy discovery.” CIHR (2010-2015); operating grant; role: Principal Investigator (PI) (2 co-applicants).
  2. “Emerging team in the Leigh Syndrome French Canadian Type (LSFC): Translating LSFC gene discovery into treatments for patients.” CIHR (2010-2015); team grant; role: Team Leader (7 co-applicants).
  3. “IBD Genomic Medicine Consortium (iGenoMed): translating genetic discoveries into a personalized approach to treating inflammatory bowel diseases.” Genome Canada (2012-2016). Role: Co-Applicant (PIs: Dr. J. Rioux and Dr. A. Bitton; 9 co-applicants); responsible for the metabolomics component.
  4. “Discovery, development and validation of biomarkers and theragnostic approaches.” CFI (2009-2014). Role: Co-Applicant (PI: Dr. J.-C. Tardif; 3 co-applicants); responsible for the metabolomics component.
Awards and distinctions

Students who work in the laboratory often win awards for their oral or poster presentations during the Montreal Heart Institute's Research Days

Most of our students also receive funding through grants awarded from various agencies.

Cécile Martel:

2013 : 1st prize for a post-doctoral poster presentation

2013-2015 :  post-doctoral award : Fonds de Recherche du Québec- Santé (FRQS)

Yvette Mukanesa:

2014: 1st prize for a master's poster presentation

2014-2015:  Student grant:  Corporation de Recherche et d'Action sur les maladies héréditaires (CORAMH)

2013-2014:  Ph.D. student grant: faculté de médecine UdeM

2013-2014:  Student grant: département de nutrition UdeM

2011-2013: Masters student grant: faculté de médecine UdeM

Marie-Eve Rivard:

2010 : 1st prize for a master’s-level oral presentation

Matthieu Ruiz:

2014: 1st prize for a post-doctoral oral presentation

2014: 1st prize for a post-doctoral seminar presentation

Julie Thompson Legault:

2012 : Student grant:  CORAMH

2011 and 2012:  Student grant: Fondation GO (now the Grand défi Pierre Lavoie)

2010-2012 : master's award: FRQS

Former students:

Roselle Gélinas:

2009-2011: Ph.D. award: Canadian Institutes of Health Research (CIHR)

Dominic Lachance:

2012 : 2nd prize for a post-doctoral poster presentation

2012-2014 : Post-doctoral award: CIHR

2010-2012 : Post-doctoral award : FRQS and Heart and Stroke Foundation of Canada (HSFC)

Benjamin Lauzier:

2011-2012:  Post-doctoral award: HSFC

Fanny Vaillant:

2012 : 1st prize for a post-doctoral oral presentation

2011 : 1st prize for a post-doctoral seminar presentation

Contact

Bertrand Bouchard
Research Assistant
514 376-3330, ext. 2133
bertrand.bouchard@mhi-rc.org

Team members

Students/post-doctoral fellows:

Sandra Therrien, BSc, M.Sc. Bioinformatics: Metabolomics and medecinal drugs response prediction in intestinal inflammatory disease: Development of a bioinformatics tool to link genes to metabolites.

Yvette Mukaneza, Ph.D. candidate nutrition, co-supervision with Dr. Lise Coderre: Impact of the mutation of the LRPPRC gene on AMP-activated protein kinase (AMPK) in the fibroblasts of patients with Leigh Syndrome French Canadian type (LSFC).

Matthieu Ruiz, PhD, Postdoctoral Fellow, co-supervision with Dr. Bruce Allen: Potential cardioprotective effect of the deletion of MAPK/MK2 in the development of diabetic cardiomyopathy.

Research assistants and associates:

Bertrand Bouchard, MSc Microbiology: Expertise in mass spectrometry (GC-MS and LC-MS/MS), including sample preparation; Email: bertrand.bouchard@mhi-rc.org

Lise Coderre, PhD Molecular Biology: Expertise in metabolic syndrome and diabetes, molecular signalling; Email: lise.coderre@umontreal.ca

Caroline Daneault, MSc Biochemistry: Expertise in mass spectrometry (GC-MS), results, data and sample analysis/preparation; Email: caroline.daneault@icm-mhi.org

Anik Forest, MSc Physics and Chemistry: Expertise in mass spectrometry (GC-MS/LC-MS/MS) and results analysis (“data mining”); Email: anik.forest@mhi-rc.org

Isabelle Robillard Frayne, MSc Chemistry: Expertise in mass spectrometry (GC-MS), results, data and sample analysis/preparation; Email: isabelle.robillard-frayne@icm-mhi.org

Julie Thompson Legault, MSc Nutrition: Expertise in metabolomics and clinical study coordination; Email: julie.thompson@mhi-rc.org

Research projects

The laboratory's main on-going research projects are funded by peer-review agencies, such as the Canadian Institutes of Health Research (CIHR), Genome Canada/Génome Québec, and the Canada Foundation for Innovation (CFI)

  1. “Metabolic investigations using mass isotopomer analysis: A paradigm for biomarker and therapy discovery.” CIHR (2010-2015); operating grant; role: Principal Investigator (PI) (2 co-applicants).
  2. “Emerging team in the Leigh Syndrome French Canadian Type (LSFC): Translating LSFC gene discovery into treatments for patients.” CIHR (2010-2015); team grant; role: Team Leader (7 co-applicants).
  3. “IBD Genomic Medicine Consortium (iGenoMed): translating genetic discoveries into a personalized approach to treating inflammatory bowel diseases.” Genome Canada (2012-2016). Role: Co-Applicant (PIs: Dr. J. Rioux and Dr. A. Bitton; 9 co-applicants); responsible for the metabolomics component.
  4. “Discovery, development and validation of biomarkers and theragnostic approaches.” CFI (2009-2014). Role: Co-Applicant (PI: Dr. J.-C. Tardif; 3 co-applicants); responsible for the metabolomics component.

Publications

Pubmed: C. Des Rosiers

Links

Association de l'Acidose lactique

Integrative genomic medicine

Awards and distinctions

Students who work in the laboratory often win awards for their oral or poster presentations during the Montreal Heart Institute's Research Days

Most of our students also receive funding through grants awarded from various agencies.

Cécile Martel:

2013 : 1st prize for a post-doctoral poster presentation

2013-2015 :  post-doctoral award : Fonds de Recherche du Québec- Santé (FRQS)

Yvette Mukanesa:

2014: 1st prize for a master's poster presentation

2014-2015:  Student grant:  Corporation de Recherche et d'Action sur les maladies héréditaires (CORAMH)

2013-2014:  Ph.D. student grant: faculté de médecine UdeM

2013-2014:  Student grant: département de nutrition UdeM

2011-2013: Masters student grant: faculté de médecine UdeM

Marie-Eve Rivard:

2010 : 1st prize for a master’s-level oral presentation

Matthieu Ruiz:

2014: 1st prize for a post-doctoral oral presentation

2014: 1st prize for a post-doctoral seminar presentation

Julie Thompson Legault:

2012 : Student grant:  CORAMH

2011 and 2012:  Student grant: Fondation GO (now the Grand défi Pierre Lavoie)

2010-2012 : master's award: FRQS

Former students:

Roselle Gélinas:

2009-2011: Ph.D. award: Canadian Institutes of Health Research (CIHR)

Dominic Lachance:

2012 : 2nd prize for a post-doctoral poster presentation

2012-2014 : Post-doctoral award: CIHR

2010-2012 : Post-doctoral award : FRQS and Heart and Stroke Foundation of Canada (HSFC)

Benjamin Lauzier:

2011-2012:  Post-doctoral award: HSFC

Fanny Vaillant:

2012 : 1st prize for a post-doctoral oral presentation

2011 : 1st prize for a post-doctoral seminar presentation

Contact

Bertrand Bouchard
Research Assistant
514 376-3330, ext. 2133
bertrand.bouchard@mhi-rc.org