Dr. Martin G. Sirois and Dr. Jean-François Tanguay have developed a histology and immunohistochemistry platform at the Montreal Heart Institute (MHI) Research Centre that is dedicated to vascular disease and associated pathologies. The goal of this platform is to promote scientific collaborations and/or service contracts with academic institutions and the pharmaceutical industry.
Dr. Sirois and Dr. Tanguay's complementary expertise in basic science and preclinical studies using various animal and clinical models has created a fertile environment for the growth of many collaborations. In recent years, through the histology and immunohistochemistry platform, Dr. Sirois and Dr. Tanguay have collaborated on over 250 projects with academic and pharma industry partners.
Le laboratoire offre tout le processus d'histologie et d'immunohistochimie.
The laboratory's services include every step of the histology and immunohistochemistry process.
Ms. Marie-Élaine Clavet-Lanthier
Ms. Marie-Élaine Clavet-Lanthier is the research technician responsible for the histology and immunohistochemistry platform. Since arriving at the laboratory in 2006, she has worked on many basic and preclinical studies. With each study, she has expanded and deepened her knowledge in the field of histology. She is also responsible for coordinating/building histological studies, preparing samples, preparing histological/immunohistochemistry staining, developing handling techniques, as well as analyzing samples.
Phone: 514-376-3330 ext. 3581
email: marie-elaine.clavet@icm-mhi.org
Martin G. Sirois, PhD, is a senior researcher at the MHI Research Centre and a full professor in the Department of Pharmacology at the Faculty of Medicine of Université de Montréal. Dr. Sirois specializes in cellular and vascular biology. His main research focus is on the role of various growth factors such as vascular endothelial growth factor (VEGF) and angiopoietins (Ang1 and Ang2) in the recruitment of circulating inflammatory cells and angiogenic processes, such as those observed in the development of atherosclerosis, solid tumour growth and other vascular pathologies.
Phone: 514-376-3330 ext. 3583
email: martin.sirois@icm-mhi.org
Dr Jean-François Tanguay, M.D.
Jean-François Tanguay, MD, is an interventional cardiologist, senior clinical research scientist at the MHI Research Centre, and associate professor in the Department of Medicine and Department of Biomedical Sciences at the Faculty of Medicine of Université de Montréal. His research looks at the pro-thrombotic and pro-inflammatory mechanisms induced during the progression of vascular diseases such as atherosclerosis or restenosis. In addition to conducting preclinical and clinical studies on antithrombotic molecules and innovative endovascular stents, Dr. Tanguay aims to develop vascular therapies that use progenitor cells and new biocompatible polymers.
Coordonnées de Dr Jean-François Tanguay
Phone: 514-376-3330 ext. 3250
email: jean-francrois.tanguay@icm-mhi.org
The team members of our histology platform work with many basic and clinical research laboratories as well as partners in the pharmaceutical and biotechnology industries. Our team also collaborates with MHI researchers and national and international partners in various biological research fields.
We provide these laboratories with specialized expertise and cutting-edge equipment in histology and immunohistochemistry :
- Sample preparation
- Paraffin/resin embedding
- Sections and section profiles
- Histology et immunohistochemistry
- Data analysis
- Expert reports
Noiseux N., Boucher C., Cartier R., Sirois M.G. A single endovascular treatment with a PDGF-β receptor antisense oligonucleotide is sufficient to suppress intimal thickening in a rat carotid injury model. Circulation, 102, 1330-1336, 2000.
Marchand G., Noiseux N., Tanguay J-F., Sirois M.G. Inhibition of VEGF-mediated angiogenesis by antisense gene therapy: role of Flk-1 and Flt-1 receptors. Am. J. Physiol., 282, H194-H204, 2002.
Khairy P., Chauvet P., Lehmann J., Lambert J., Macle L., Tanguay J-F., Sirois M.G., Santoianni D., Dubuc M. Lower incidence of thrombus formation with cryoenergy versus radiofrequency catheter ablation. Circulation, 107, 2045-2050, 2003.
Tanguay J.-F., Goeffroy P., Sirois M.G., Libersan D., Kumar A., Schaub R.G., Merhi Y. Prevention of in-stent restenosis via reduction of thrombo-inflammatory reactions with recombinant P-selectin glycoprotein ligand-1. Thrombosis Haemostasis, 91, 1186-1193, 2004.
Tanguay J-F., Goeffroy P., Dorval J-F., Sirois M.G. Percutaneous endoluminal arterial cryoenergy improves vascular remodeling after angioplasty. Thrombosis Haemostasis, 92, 1114-1121, 2004.
Guerra PG, Talajic M, Thibault B, Dubuc M, Roy D, Macle L, Leung TK, Arsenault A, Harel F, Grégoire J, Bonan R. Beta-radiation for the creation of linear lesions in the canine atrium. Circulation. 110, 911-914, 2004.
Chandrasekar B., Sirois M.G., Nattel S., Tanguay J.-F. Local delivery of 17-estradiol improves reendothelialization and decreases inflammation after coronary stenting in a porcine model. Thrombosis & Haemostatis. 94, 1042-1047, 2005.
Favier J., Lapointe S., Maliba R., Sirois M.G. HIF2a/EPAS1 promotes the formation of slow-growing but highly angiogenic tumors in a mouse model of neuroblastoma. Biomed central – Cancer. 7, 1-10, 2007.
Busseuil D., Shi Y., Mecteau M., Brand G., Kernaleguen A.E., Thorin E., Latour J.G., Rhéaume E., Tardif J.C. Regression of aortic valve stenosis by ApoA-I mimetic peptide infusions in rabbits. Br. J. Pharmacol., 155, 1163-1170, 2008.
Haddad L., Bou Khzam L., Hajjar F., Merhi Y., Sirois M.G. Characterization of FGF receptors expression on human neutrophils and their contribution to chemotaxis. Am. J. Physiol. Cell Physiol. 301, C1036-45, 2011.
The Histology and Immunohistochemistry Laboratory was created thanks to grants received through the CFI Infrastructure Operating Fund between 1998 and 2010.
Team members
Ms. Marie-Élaine Clavet-Lanthier
Ms. Marie-Élaine Clavet-Lanthier is the research technician responsible for the histology and immunohistochemistry platform. Since arriving at the laboratory in 2006, she has worked on many basic and preclinical studies. With each study, she has expanded and deepened her knowledge in the field of histology. She is also responsible for coordinating/building histological studies, preparing samples, preparing histological/immunohistochemistry staining, developing handling techniques, as well as analyzing samples.
Phone: 514-376-3330 ext. 3581
email: marie-elaine.clavet@icm-mhi.org
Martin G. Sirois, PhD, is a senior researcher at the MHI Research Centre and a full professor in the Department of Pharmacology at the Faculty of Medicine of Université de Montréal. Dr. Sirois specializes in cellular and vascular biology. His main research focus is on the role of various growth factors such as vascular endothelial growth factor (VEGF) and angiopoietins (Ang1 and Ang2) in the recruitment of circulating inflammatory cells and angiogenic processes, such as those observed in the development of atherosclerosis, solid tumour growth and other vascular pathologies.
Phone: 514-376-3330 ext. 3583
email: martin.sirois@icm-mhi.org
Dr Jean-François Tanguay, M.D.
Jean-François Tanguay, MD, is an interventional cardiologist, senior clinical research scientist at the MHI Research Centre, and associate professor in the Department of Medicine and Department of Biomedical Sciences at the Faculty of Medicine of Université de Montréal. His research looks at the pro-thrombotic and pro-inflammatory mechanisms induced during the progression of vascular diseases such as atherosclerosis or restenosis. In addition to conducting preclinical and clinical studies on antithrombotic molecules and innovative endovascular stents, Dr. Tanguay aims to develop vascular therapies that use progenitor cells and new biocompatible polymers.
Coordonnées de Dr Jean-François Tanguay
Phone: 514-376-3330 ext. 3250
email: jean-francrois.tanguay@icm-mhi.org
Research projects
The team members of our histology platform work with many basic and clinical research laboratories as well as partners in the pharmaceutical and biotechnology industries. Our team also collaborates with MHI researchers and national and international partners in various biological research fields.
We provide these laboratories with specialized expertise and cutting-edge equipment in histology and immunohistochemistry :
- Sample preparation
- Paraffin/resin embedding
- Sections and section profiles
- Histology et immunohistochemistry
- Data analysis
- Expert reports
Publications
Noiseux N., Boucher C., Cartier R., Sirois M.G. A single endovascular treatment with a PDGF-β receptor antisense oligonucleotide is sufficient to suppress intimal thickening in a rat carotid injury model. Circulation, 102, 1330-1336, 2000.
Marchand G., Noiseux N., Tanguay J-F., Sirois M.G. Inhibition of VEGF-mediated angiogenesis by antisense gene therapy: role of Flk-1 and Flt-1 receptors. Am. J. Physiol., 282, H194-H204, 2002.
Khairy P., Chauvet P., Lehmann J., Lambert J., Macle L., Tanguay J-F., Sirois M.G., Santoianni D., Dubuc M. Lower incidence of thrombus formation with cryoenergy versus radiofrequency catheter ablation. Circulation, 107, 2045-2050, 2003.
Tanguay J.-F., Goeffroy P., Sirois M.G., Libersan D., Kumar A., Schaub R.G., Merhi Y. Prevention of in-stent restenosis via reduction of thrombo-inflammatory reactions with recombinant P-selectin glycoprotein ligand-1. Thrombosis Haemostasis, 91, 1186-1193, 2004.
Tanguay J-F., Goeffroy P., Dorval J-F., Sirois M.G. Percutaneous endoluminal arterial cryoenergy improves vascular remodeling after angioplasty. Thrombosis Haemostasis, 92, 1114-1121, 2004.
Guerra PG, Talajic M, Thibault B, Dubuc M, Roy D, Macle L, Leung TK, Arsenault A, Harel F, Grégoire J, Bonan R. Beta-radiation for the creation of linear lesions in the canine atrium. Circulation. 110, 911-914, 2004.
Chandrasekar B., Sirois M.G., Nattel S., Tanguay J.-F. Local delivery of 17-estradiol improves reendothelialization and decreases inflammation after coronary stenting in a porcine model. Thrombosis & Haemostatis. 94, 1042-1047, 2005.
Favier J., Lapointe S., Maliba R., Sirois M.G. HIF2a/EPAS1 promotes the formation of slow-growing but highly angiogenic tumors in a mouse model of neuroblastoma. Biomed central – Cancer. 7, 1-10, 2007.
Busseuil D., Shi Y., Mecteau M., Brand G., Kernaleguen A.E., Thorin E., Latour J.G., Rhéaume E., Tardif J.C. Regression of aortic valve stenosis by ApoA-I mimetic peptide infusions in rabbits. Br. J. Pharmacol., 155, 1163-1170, 2008.
Haddad L., Bou Khzam L., Hajjar F., Merhi Y., Sirois M.G. Characterization of FGF receptors expression on human neutrophils and their contribution to chemotaxis. Am. J. Physiol. Cell Physiol. 301, C1036-45, 2011.
Links
Awards and Recognition
The Histology and Immunohistochemistry Laboratory was created thanks to grants received through the CFI Infrastructure Operating Fund between 1998 and 2010.